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    Structured Review

    R&D Systems cf 119 bp r d system minneapolis
    Cf 119 Bp R D System Minneapolis, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cf+r+d+systems/Recombinant+Human+IL-18+BPa+Fc+Chimera+Protein%2C+CF/pm42010420-58-60-62
    Average 93 stars, based on 28 article reviews
    cf 119 bp r d system minneapolis - by Bioz Stars, 2026-09
    93/100 stars

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    Virus:

    Article Title: tRNA-overlapping long non-coding RNA loci repress codon-biased genes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Collagen Type IIA Leica Cat# NCL-COLL-IIp; RRID:AB_563666 CD105 Invitrogen Cat# MHCD10504; RRID:AB_10392382 CD90 BD Biosciences Cat#555595 CD73 BD Biosciences Cat#CD73 HLA-ABC BD Biosciences Cat#555552 CD31 BD Biosciences Cat#555445 CD34 BD Biosciences Cat#555821 CD45 BD Biosciences Cat#555482 eIF2α polyclonal antibody Bethyl Laboratories Cat#A301-949A-T Phospho-eIF2α Abcam Cat# ab32157; RRID:AB_732117 HLA-DR BD Biosciences Cat#555561 Goat Anti-Rabbit IgG Antibody, HRP-conjugate Sigma-Aldrich Cat#12-348; RRID:AB_390191 α-Tubulin Rabbit mAb CST Cat#2125S; RRID:AB_2619646 Bacterial and virus strains NEB 5-alpha Competent E.coli (High Efficiency) NEB Cat#C2987H Biological samples Primary Mesenchymal Stem Cells lipoaspirates N/A Chemicals, peptides, and recombinant proteins L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate Millipore Sigma Cat#A8960 Pyruvic acid sodium cell culture tested Millipore Sigma Cat#P5280-25G Dexamethasone bioreagent Millipore Sigma Cat#D4902-100MG Insulin-Transferrin-Selenium-Ethanolamine (100X) Gibco Cat#51500056 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich Cat# H3149 Concentrated Thrombocytes Buffy coat N/A Cycloheximide Sigma-Aldrich Cat#C1988-1G Indomethacin Sigma-Aldrich Cat#17378 IBMX Sigma-Aldrich Cat# I5879 Insulin Sigma-Aldrich Cat#19278 Oil red Thermo Scientific Cat# A12989-22 rhBMP2 R&D Systems Cat#355-BEC-010/CF β-glycerophosphate Sigma-Aldrich Cat# G9422 Alizarin Red S Sigma-Aldrich Cat# A5533 Recombinant Human IGF-I/IGF-1 Protein, CF R&D Systems Cat291-G1-200 Recombinant Human TGF-beta 1 Protein R&D Systems Cat#240-B-002 Toluidine Blue O Sigma-Aldrich Cat# T3260 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific Cat#28908 Alcian blue Sigma-Aldrich Cat#66011 Sirius Red Sigma-Aldrich Cat#365548 Acetic acid Millipore Sigma Cat#A6283-500ML Paraffin Supelco Cat#1.07160 DAPI Sigma-Aldrich Cat#D9542 Trizol Reagent Life Technologies Cat#15596018 (Continued on next page) Cell Reports 44, 116417, October 28, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER DNase I, Amplification Grade Invitrogen Cat#18068015 Recombinant Human FGF basic (146 aa) Protein R&D Systems Cat#233-FB-010 Recombinant Human FGF acidic (aa 16–155) Protein R&D Systems Cat#232-FA-025 Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG-200 L-Alanyl-L-glutamine Thermo Scientific Chemicals 39537-23-0 Geneticin Gibco Cat#10131035 BbsI-HF NEB Cat#R3539L KpnI-HF NEB Cat#R3142L XbaI NEB Cat#R0145L Lipofectamine TM 3000 Transfection Reagent Invitrogen Cat#L3000075 Puromycin Dihydrochloride Gibco Cat#A1113803 DirectPCR Lysis Reagent (Cell) Viagen Biotech Cat#301-C OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer NEB Cat#M0488L PowerUp TM SYBR TM Green Master Mix ABI Cat#A25742 ATCC Mesenchymal Stem Cell Basal Medium Cedarlane Cat#PCS-500-030 Sodium Bicarbonate Gibco Cat#25080094 DMEM/F-12, GlutaMAX TM supplement Gibco 10565042 Critical commercial assays TruSeq Stranded Total RNA Ribo-Zero Gold Illumina Cat#RS-122-2301 Monarch DNA Gel Extraction Kit NEB Cat#T1020L SuperScript III 1st Strand Synthesis Invitrogen Cat#18080051 TruSeq Stranded Total RNA Ribo-Zero Gold Illumina Cat#RS-122-2301 BCA protein concentration assay Pierce Cat#23225 Deposited data RNAseq time course of primary mesenchymal stem cells (MSCs) differentiated into different stages of chondrogenesis (male & female), RNAseq tROL and lncRNA knockouts in C28/I-2 and HEK-293 cells, and SCRC-4000 chondrogenic time course.


    Recombinant:

    Article Title: tRNA-overlapping long non-coding RNA loci repress codon-biased genes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Collagen Type IIA Leica Cat# NCL-COLL-IIp; RRID:AB_563666 CD105 Invitrogen Cat# MHCD10504; RRID:AB_10392382 CD90 BD Biosciences Cat#555595 CD73 BD Biosciences Cat#CD73 HLA-ABC BD Biosciences Cat#555552 CD31 BD Biosciences Cat#555445 CD34 BD Biosciences Cat#555821 CD45 BD Biosciences Cat#555482 eIF2α polyclonal antibody Bethyl Laboratories Cat#A301-949A-T Phospho-eIF2α Abcam Cat# ab32157; RRID:AB_732117 HLA-DR BD Biosciences Cat#555561 Goat Anti-Rabbit IgG Antibody, HRP-conjugate Sigma-Aldrich Cat#12-348; RRID:AB_390191 α-Tubulin Rabbit mAb CST Cat#2125S; RRID:AB_2619646 Bacterial and virus strains NEB 5-alpha Competent E.coli (High Efficiency) NEB Cat#C2987H Biological samples Primary Mesenchymal Stem Cells lipoaspirates N/A Chemicals, peptides, and recombinant proteins L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate Millipore Sigma Cat#A8960 Pyruvic acid sodium cell culture tested Millipore Sigma Cat#P5280-25G Dexamethasone bioreagent Millipore Sigma Cat#D4902-100MG Insulin-Transferrin-Selenium-Ethanolamine (100X) Gibco Cat#51500056 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich Cat# H3149 Concentrated Thrombocytes Buffy coat N/A Cycloheximide Sigma-Aldrich Cat#C1988-1G Indomethacin Sigma-Aldrich Cat#17378 IBMX Sigma-Aldrich Cat# I5879 Insulin Sigma-Aldrich Cat#19278 Oil red Thermo Scientific Cat# A12989-22 rhBMP2 R&D Systems Cat#355-BEC-010/CF β-glycerophosphate Sigma-Aldrich Cat# G9422 Alizarin Red S Sigma-Aldrich Cat# A5533 Recombinant Human IGF-I/IGF-1 Protein, CF R&D Systems Cat291-G1-200 Recombinant Human TGF-beta 1 Protein R&D Systems Cat#240-B-002 Toluidine Blue O Sigma-Aldrich Cat# T3260 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific Cat#28908 Alcian blue Sigma-Aldrich Cat#66011 Sirius Red Sigma-Aldrich Cat#365548 Acetic acid Millipore Sigma Cat#A6283-500ML Paraffin Supelco Cat#1.07160 DAPI Sigma-Aldrich Cat#D9542 Trizol Reagent Life Technologies Cat#15596018 (Continued on next page) Cell Reports 44, 116417, October 28, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER DNase I, Amplification Grade Invitrogen Cat#18068015 Recombinant Human FGF basic (146 aa) Protein R&D Systems Cat#233-FB-010 Recombinant Human FGF acidic (aa 16–155) Protein R&D Systems Cat#232-FA-025 Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG-200 L-Alanyl-L-glutamine Thermo Scientific Chemicals 39537-23-0 Geneticin Gibco Cat#10131035 BbsI-HF NEB Cat#R3539L KpnI-HF NEB Cat#R3142L XbaI NEB Cat#R0145L Lipofectamine TM 3000 Transfection Reagent Invitrogen Cat#L3000075 Puromycin Dihydrochloride Gibco Cat#A1113803 DirectPCR Lysis Reagent (Cell) Viagen Biotech Cat#301-C OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer NEB Cat#M0488L PowerUp TM SYBR TM Green Master Mix ABI Cat#A25742 ATCC Mesenchymal Stem Cell Basal Medium Cedarlane Cat#PCS-500-030 Sodium Bicarbonate Gibco Cat#25080094 DMEM/F-12, GlutaMAX TM supplement Gibco 10565042 Critical commercial assays TruSeq Stranded Total RNA Ribo-Zero Gold Illumina Cat#RS-122-2301 Monarch DNA Gel Extraction Kit NEB Cat#T1020L SuperScript III 1st Strand Synthesis Invitrogen Cat#18080051 TruSeq Stranded Total RNA Ribo-Zero Gold Illumina Cat#RS-122-2301 BCA protein concentration assay Pierce Cat#23225 Deposited data RNAseq time course of primary mesenchymal stem cells (MSCs) differentiated into different stages of chondrogenesis (male & female), RNAseq tROL and lncRNA knockouts in C28/I-2 and HEK-293 cells, and SCRC-4000 chondrogenic time course.


    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Article Title: Laminin-defined mechanical status modulates retinal pigment epithelium phagocytosis.
    Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number KnockOut Serum Thermo Fisher Scientific Cat #10828-028 MEM α, GlutaMAXTM Supplement, no nucleosides Gibco Cat #32561-029 N-2 supplement Gibco Cat #17502048 Recombinant Human MFG-E8 Protein, CF R&D systems Cat #2767-MF-050 Paraformaldehyde, prilled, 95% Sigma-Aldrich Cat #441244-1KG Taurine Sigma-Aldrich Cat #T0625 TrypLE Express dissociation agent Gibco Cat #12605-010 Trypsin-EDTA solution Sigma-Aldrich Cat #T4049-500ML Inhibitors RHO ACTIVATOR II Cytoskeleton Cat #CN03-A ROCK-Inhibitor (Y-27632) Sigma-Aldrich Cat #0503-1MG Extracellular matrix proteins Biolaminin 211 BioLamina Cat #LN211-02 Biolaminin 332 BioLamina Cat #LN332-0502 Biolaminin 511 BioLamina Cat #LN511-0502 Human Collagen Type IV, Collagen from human placenta Sigma-Aldrich Cat #C7521-10MG Vitronectin XF StemCell Technologies Cat #07180 Software GraphPad Prism 10.4 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html DataViewer V2.4.0 https:// www.optics11life.com FlowJo https:// www.flowjo.com Cellpose https:// www.cellpose.org MATLAB https:// www.mathworks.com/ products/matlab.html Affinity Designer https:// affinity.serif.com Other Confocal LSM 710 Carl ZEISS AG Axio Observer 7 Inverted Microscope Carl ZEISS AG ApoTome.2 Fluorescence Microscope Carl ZEISS AG Chiaro Nanoindenter Optics11 Life LSRFortessa Cell Analyser BD Biosciences Dimension Icon Atomic Force Microscope Bruker ..

    Cell Culture:

    Article Title: tRNA-overlapping long non-coding RNA loci repress codon-biased genes.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Collagen Type IIA Leica Cat# NCL-COLL-IIp; RRID:AB_563666 CD105 Invitrogen Cat# MHCD10504; RRID:AB_10392382 CD90 BD Biosciences Cat#555595 CD73 BD Biosciences Cat#CD73 HLA-ABC BD Biosciences Cat#555552 CD31 BD Biosciences Cat#555445 CD34 BD Biosciences Cat#555821 CD45 BD Biosciences Cat#555482 eIF2α polyclonal antibody Bethyl Laboratories Cat#A301-949A-T Phospho-eIF2α Abcam Cat# ab32157; RRID:AB_732117 HLA-DR BD Biosciences Cat#555561 Goat Anti-Rabbit IgG Antibody, HRP-conjugate Sigma-Aldrich Cat#12-348; RRID:AB_390191 α-Tubulin Rabbit mAb CST Cat#2125S; RRID:AB_2619646 Bacterial and virus strains NEB 5-alpha Competent E.coli (High Efficiency) NEB Cat#C2987H Biological samples Primary Mesenchymal Stem Cells lipoaspirates N/A Chemicals, peptides, and recombinant proteins L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate Millipore Sigma Cat#A8960 Pyruvic acid sodium cell culture tested Millipore Sigma Cat#P5280-25G Dexamethasone bioreagent Millipore Sigma Cat#D4902-100MG Insulin-Transferrin-Selenium-Ethanolamine (100X) Gibco Cat#51500056 Heparin sodium salt from porcine intestinal mucosa Sigma-Aldrich Cat# H3149 Concentrated Thrombocytes Buffy coat N/A Cycloheximide Sigma-Aldrich Cat#C1988-1G Indomethacin Sigma-Aldrich Cat#17378 IBMX Sigma-Aldrich Cat# I5879 Insulin Sigma-Aldrich Cat#19278 Oil red Thermo Scientific Cat# A12989-22 rhBMP2 R&D Systems Cat#355-BEC-010/CF β-glycerophosphate Sigma-Aldrich Cat# G9422 Alizarin Red S Sigma-Aldrich Cat# A5533 Recombinant Human IGF-I/IGF-1 Protein, CF R&D Systems Cat291-G1-200 Recombinant Human TGF-beta 1 Protein R&D Systems Cat#240-B-002 Toluidine Blue O Sigma-Aldrich Cat# T3260 Pierce TM 16% Formaldehyde (w/v), Methanol-free Thermo Scientific Cat#28908 Alcian blue Sigma-Aldrich Cat#66011 Sirius Red Sigma-Aldrich Cat#365548 Acetic acid Millipore Sigma Cat#A6283-500ML Paraffin Supelco Cat#1.07160 DAPI Sigma-Aldrich Cat#D9542 Trizol Reagent Life Technologies Cat#15596018 (Continued on next page) Cell Reports 44, 116417, October 28, 2025 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER DNase I, Amplification Grade Invitrogen Cat#18068015 Recombinant Human FGF basic (146 aa) Protein R&D Systems Cat#233-FB-010 Recombinant Human FGF acidic (aa 16–155) Protein R&D Systems Cat#232-FA-025 Recombinant Human EGF Protein, CF R&D Systems Cat#236-EG-200 L-Alanyl-L-glutamine Thermo Scientific Chemicals 39537-23-0 Geneticin Gibco Cat#10131035 BbsI-HF NEB Cat#R3539L KpnI-HF NEB Cat#R3142L XbaI NEB Cat#R0145L Lipofectamine TM 3000 Transfection Reagent Invitrogen Cat#L3000075 Puromycin Dihydrochloride Gibco Cat#A1113803 DirectPCR Lysis Reagent (Cell) Viagen Biotech Cat#301-C OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer NEB Cat#M0488L PowerUp TM SYBR TM Green Master Mix ABI Cat#A25742 ATCC Mesenchymal Stem Cell Basal Medium Cedarlane Cat#PCS-500-030 Sodium Bicarbonate Gibco Cat#25080094 DMEM/F-12, GlutaMAX TM supplement Gibco 10565042 Critical commercial assays TruSeq Stranded Total RNA Ribo-Zero Gold Illumina Cat#RS-122-2301 Monarch DNA Gel Extraction Kit NEB Cat#T1020L SuperScript III 1st Strand Synthesis Invitrogen Cat#18080051 TruSeq Stranded Total RNA Ribo-Zero Gold Illumina Cat#RS-122-2301 BCA protein concentration assay Pierce Cat#23225 Deposited data RNAseq time course of primary mesenchymal stem cells (MSCs) differentiated into different stages of chondrogenesis (male & female), RNAseq tROL and lncRNA knockouts in C28/I-2 and HEK-293 cells, and SCRC-4000 chondrogenic time course.

    Activity Assay:


    Clinical Proteomics:

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Produced:

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Staining:

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Cell Isolation:

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Labeling:

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Bicinchoninic Acid Protein Assay:

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Protease Inhibitor:

    Article Title: A type I interferon regulatory network for human plasmacytoid dendritic cells based on heparin, membrane-bound and soluble BDCA-2
    Article Snippet: Reagents Regaent Source Identifier Streptavidin-POD Roche Cat# 11089153001 Human serum defibrinated, AB Sigma-Aldrich Cat# H4522 DMEM PAN-Biotech Cat# P04-03600 RPMI 1640 PAN-Biotech Cat# P04-18000 Lymphocyte separation media Anprotec Cat# MS01AE 1001 OptiMem Gibco Cat# 31985070 L-Glutamine PAN Biotech Cat# P04-80100 Penicillin-Streptomycin PAN Biotech Cat# P06-07100 Fetal calf serum (FCS) Sigma-Aldrich Cat# F7524 MEM non-essential amino acids solution (100x) Gibco Cat# 11140050 2-mercaptoethanol Gibco Cat# 31,350-010 Sodium pyruvate Gibco Cat# 11360070 Soluble human BDCA-2 This manuscript N/A Soluble human DCIR This manuscript N/A Soluble human Dectin-2 This manuscript N/A Soluble human IL-8 This manuscript N/A Human IL-6 ImmunoTools Cat# 11340064 Amonium sulfate ROTH Cat# 3746.1 Chondroitin Sigma-Aldrich Cat# C9819 Dermatan Sigma-Aldrich Cat# C3788 Heparan sulfate Iduron Cat# GAG-HS-I Heparin 25000 I.E./5 mL Panpharma N/A Fondaparinux 1,5 mg/0,3 mL aspen N/A Enoxaparin (Clexane) 40 mg/0,4 mL SANOFI N/A Dalteparin (Fragmin) 2500 IE/0,2 mL Pfizer N/A Hyaluronic acid Sigma-Aldrich Cat# 924474 HiTrap® Heparin High Performance Cytiva 17-0406-01, pack of 5 × 1 mL Sigma-Aldrich Cat# GE17-0406-01 Ionomycin calcium salt from Streptomyces conglobatus Sigma-Aldrich Cat# I0634 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich Cat# I0634 Strep-Tactin®XT 4Flow® cartridge IBA Cat# 2-5023-001 Biotin IBA Cat# 2-1016-005 GW 280264X Cayman Cat# 31388 GI 254023X Cayman Cat# 28284 GeneticinTM selektives Antibiotikum (G418-Sulfat) (50 mg/mL) Gibco Cat# 10131035 Supersignal west femto Maximum sensitivity substrate Thermo Fisher Scientific Cat# 34095 Super signal west Dura trial kit Thermo Fisher Scientific Cat# 37071 GL Sciences Titansphere (TiO2) 100Å 5μm, Bulk Material, 500mg, ea. .. GL Sciences Inc. Cat# GL-5020- 75000 IMDM, w/o: L-Glutamine, w: 25 mM HEPES, w: 3.024 g/L NaHCO3 PAN BIOTECH Cat# P04-20050 Insulin-Transferrin-Selenium (ITS -G) (100X) GIBCO Cat# 41400045 Table S3. (continued) MEM Vitamin Solution (100X) GIBCO Cat# 11120037 MEM Amino Acids Solution (50X) GIBCO Cat# 11130036 rmSCF Markus Schnare, Germany N/A Albumin from human serum Sigma-Aldrich Cat# A3782-100MG Fibrinogen from human plasma Sigma-Aldrich Cat# F3879-100MG Bio-Pure Human Serum Albumin (HSA) 10% solution Sartorius Cat# 05-720-1D Recombinant P. vulgaris Chondroitinase ABC Protein, CF R&D systems Cat# 6877-GH-020 Bacteroides Heparinase I New england biolabs Cat# P0735S Bacteroides Heparinase II New england biolabs Cat# P0736S Bacteroides Heparinase III New england biolabs Cat# P0737S Perchloric acid ACS reagent, 70% Sigma-Aldrich Cat# 244252-100ML 4–20% Mini-PROTEAN® TGXTM Precast Protein Gels, 12-well, 20 μl #4561095 Biorad Cat# 4561095 Toluidine Blue O Sigma-Aldrich Cat# T3260 Flt3-L Supernatant produced within Department for Immunology N/A PageBlueTM Protein Staining Solution Thermo Fisher Scientific Cat# 24620 mIFN-α Supernatant produced within Department for Immunology N/A Recombinant Mouse M-CSF Protein R&D systems Cat# 416-ML Amicon Ultra - 4 Millipore Cat# UFC801024 Strep-Tactin® HRP conjugate IBA Cat# 2-1502-001 DAPI Sigma-Aldrich Cat# D9542 Perchloric acid Honeywell Cat# 244252 Ammonium hydroxide solution Honeywell Cat# 221228 Critical commercial assays Plasmacytoid Dendritic Cell Isolation Kit II Miltenyi Biotec Cat# 130-097-415 Human DLEC/CLEC4C/BDCA2 ELISA Kit Invitrogen Cat# EH156RB Anti IIa Assay Kit Kinetichrome Cat# IIAI IFN alpha Human Matched Antibody Pair Invitrogen Cat# BMS216MST Protein Labeling Kit RED-MALEIMIDE 2nd Generation (Cysteine reactive) Nanotemper Cat# MO-L014 Pierce BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23225 Protease inhibitor Cocktail kit Thermo Fisher Scientific Cat# 78410 2216 CpG 20-mer TIB MOLBIOL N/A CpG2216_Cy3 Biospring Cat# 290536_G pcDNA3.1(-)_sol-hBDCA2 This work GenBank accession# OQ817991 Table S3. (continued) pcDNA3.1(-)_sol-hBDCA2_N110Q This work GenBank accession# OQ817992 pcDNA3.1(-)_sol-hBDCA2_N137Q This work GenBank accession# OQ817993 pcDNA3.1(-)_sol-hBDCA2_N164Q _ This work GenBank accession# OQ817994 pcDNA3.1(-)_sol-hBDCA2_E172A/N174A This work GenBank accession# OQ817995 pcDNA3.1(-)_sol-hDCIR This work GenBank accession# OQ817996 pcDNA3.1(-)_sol-hDECTIN-2 This work GenBank accession# OQ817997 pcDNA3.1(-)_sol-hIL-8 This work GenBank accession# OQ817998 ..

    Knock-Out:

    Article Title: Laminin-defined mechanical status modulates retinal pigment epithelium phagocytosis.
    Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number KnockOut Serum Thermo Fisher Scientific Cat #10828-028 MEM α, GlutaMAXTM Supplement, no nucleosides Gibco Cat #32561-029 N-2 supplement Gibco Cat #17502048 Recombinant Human MFG-E8 Protein, CF R&D systems Cat #2767-MF-050 Paraformaldehyde, prilled, 95% Sigma-Aldrich Cat #441244-1KG Taurine Sigma-Aldrich Cat #T0625 TrypLE Express dissociation agent Gibco Cat #12605-010 Trypsin-EDTA solution Sigma-Aldrich Cat #T4049-500ML Inhibitors RHO ACTIVATOR II Cytoskeleton Cat #CN03-A ROCK-Inhibitor (Y-27632) Sigma-Aldrich Cat #0503-1MG Extracellular matrix proteins Biolaminin 211 BioLamina Cat #LN211-02 Biolaminin 332 BioLamina Cat #LN332-0502 Biolaminin 511 BioLamina Cat #LN511-0502 Human Collagen Type IV, Collagen from human placenta Sigma-Aldrich Cat #C7521-10MG Vitronectin XF StemCell Technologies Cat #07180 Software GraphPad Prism 10.4 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html DataViewer V2.4.0 https:// www.optics11life.com FlowJo https:// www.flowjo.com Cellpose https:// www.cellpose.org MATLAB https:// www.mathworks.com/ products/matlab.html Affinity Designer https:// affinity.serif.com Other Confocal LSM 710 Carl ZEISS AG Axio Observer 7 Inverted Microscope Carl ZEISS AG ApoTome.2 Fluorescence Microscope Carl ZEISS AG Chiaro Nanoindenter Optics11 Life LSRFortessa Cell Analyser BD Biosciences Dimension Icon Atomic Force Microscope Bruker ..

    Software:

    Article Title: Laminin-defined mechanical status modulates retinal pigment epithelium phagocytosis.
    Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number KnockOut Serum Thermo Fisher Scientific Cat #10828-028 MEM α, GlutaMAXTM Supplement, no nucleosides Gibco Cat #32561-029 N-2 supplement Gibco Cat #17502048 Recombinant Human MFG-E8 Protein, CF R&D systems Cat #2767-MF-050 Paraformaldehyde, prilled, 95% Sigma-Aldrich Cat #441244-1KG Taurine Sigma-Aldrich Cat #T0625 TrypLE Express dissociation agent Gibco Cat #12605-010 Trypsin-EDTA solution Sigma-Aldrich Cat #T4049-500ML Inhibitors RHO ACTIVATOR II Cytoskeleton Cat #CN03-A ROCK-Inhibitor (Y-27632) Sigma-Aldrich Cat #0503-1MG Extracellular matrix proteins Biolaminin 211 BioLamina Cat #LN211-02 Biolaminin 332 BioLamina Cat #LN332-0502 Biolaminin 511 BioLamina Cat #LN511-0502 Human Collagen Type IV, Collagen from human placenta Sigma-Aldrich Cat #C7521-10MG Vitronectin XF StemCell Technologies Cat #07180 Software GraphPad Prism 10.4 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html DataViewer V2.4.0 https:// www.optics11life.com FlowJo https:// www.flowjo.com Cellpose https:// www.cellpose.org MATLAB https:// www.mathworks.com/ products/matlab.html Affinity Designer https:// affinity.serif.com Other Confocal LSM 710 Carl ZEISS AG Axio Observer 7 Inverted Microscope Carl ZEISS AG ApoTome.2 Fluorescence Microscope Carl ZEISS AG Chiaro Nanoindenter Optics11 Life LSRFortessa Cell Analyser BD Biosciences Dimension Icon Atomic Force Microscope Bruker ..

    Inverted Microscopy:

    Article Title: Laminin-defined mechanical status modulates retinal pigment epithelium phagocytosis.
    Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number KnockOut Serum Thermo Fisher Scientific Cat #10828-028 MEM α, GlutaMAXTM Supplement, no nucleosides Gibco Cat #32561-029 N-2 supplement Gibco Cat #17502048 Recombinant Human MFG-E8 Protein, CF R&D systems Cat #2767-MF-050 Paraformaldehyde, prilled, 95% Sigma-Aldrich Cat #441244-1KG Taurine Sigma-Aldrich Cat #T0625 TrypLE Express dissociation agent Gibco Cat #12605-010 Trypsin-EDTA solution Sigma-Aldrich Cat #T4049-500ML Inhibitors RHO ACTIVATOR II Cytoskeleton Cat #CN03-A ROCK-Inhibitor (Y-27632) Sigma-Aldrich Cat #0503-1MG Extracellular matrix proteins Biolaminin 211 BioLamina Cat #LN211-02 Biolaminin 332 BioLamina Cat #LN332-0502 Biolaminin 511 BioLamina Cat #LN511-0502 Human Collagen Type IV, Collagen from human placenta Sigma-Aldrich Cat #C7521-10MG Vitronectin XF StemCell Technologies Cat #07180 Software GraphPad Prism 10.4 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html DataViewer V2.4.0 https:// www.optics11life.com FlowJo https:// www.flowjo.com Cellpose https:// www.cellpose.org MATLAB https:// www.mathworks.com/ products/matlab.html Affinity Designer https:// affinity.serif.com Other Confocal LSM 710 Carl ZEISS AG Axio Observer 7 Inverted Microscope Carl ZEISS AG ApoTome.2 Fluorescence Microscope Carl ZEISS AG Chiaro Nanoindenter Optics11 Life LSRFortessa Cell Analyser BD Biosciences Dimension Icon Atomic Force Microscope Bruker ..

    Fluorescence:

    Article Title: Laminin-defined mechanical status modulates retinal pigment epithelium phagocytosis.
    Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number KnockOut Serum Thermo Fisher Scientific Cat #10828-028 MEM α, GlutaMAXTM Supplement, no nucleosides Gibco Cat #32561-029 N-2 supplement Gibco Cat #17502048 Recombinant Human MFG-E8 Protein, CF R&D systems Cat #2767-MF-050 Paraformaldehyde, prilled, 95% Sigma-Aldrich Cat #441244-1KG Taurine Sigma-Aldrich Cat #T0625 TrypLE Express dissociation agent Gibco Cat #12605-010 Trypsin-EDTA solution Sigma-Aldrich Cat #T4049-500ML Inhibitors RHO ACTIVATOR II Cytoskeleton Cat #CN03-A ROCK-Inhibitor (Y-27632) Sigma-Aldrich Cat #0503-1MG Extracellular matrix proteins Biolaminin 211 BioLamina Cat #LN211-02 Biolaminin 332 BioLamina Cat #LN332-0502 Biolaminin 511 BioLamina Cat #LN511-0502 Human Collagen Type IV, Collagen from human placenta Sigma-Aldrich Cat #C7521-10MG Vitronectin XF StemCell Technologies Cat #07180 Software GraphPad Prism 10.4 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html DataViewer V2.4.0 https:// www.optics11life.com FlowJo https:// www.flowjo.com Cellpose https:// www.cellpose.org MATLAB https:// www.mathworks.com/ products/matlab.html Affinity Designer https:// affinity.serif.com Other Confocal LSM 710 Carl ZEISS AG Axio Observer 7 Inverted Microscope Carl ZEISS AG ApoTome.2 Fluorescence Microscope Carl ZEISS AG Chiaro Nanoindenter Optics11 Life LSRFortessa Cell Analyser BD Biosciences Dimension Icon Atomic Force Microscope Bruker ..

    Microscopy:

    Article Title: Laminin-defined mechanical status modulates retinal pigment epithelium phagocytosis.
    Article Snippet: .. Reagent/Resource Reference or Source Identifier or Catalog Number KnockOut Serum Thermo Fisher Scientific Cat #10828-028 MEM α, GlutaMAXTM Supplement, no nucleosides Gibco Cat #32561-029 N-2 supplement Gibco Cat #17502048 Recombinant Human MFG-E8 Protein, CF R&D systems Cat #2767-MF-050 Paraformaldehyde, prilled, 95% Sigma-Aldrich Cat #441244-1KG Taurine Sigma-Aldrich Cat #T0625 TrypLE Express dissociation agent Gibco Cat #12605-010 Trypsin-EDTA solution Sigma-Aldrich Cat #T4049-500ML Inhibitors RHO ACTIVATOR II Cytoskeleton Cat #CN03-A ROCK-Inhibitor (Y-27632) Sigma-Aldrich Cat #0503-1MG Extracellular matrix proteins Biolaminin 211 BioLamina Cat #LN211-02 Biolaminin 332 BioLamina Cat #LN332-0502 Biolaminin 511 BioLamina Cat #LN511-0502 Human Collagen Type IV, Collagen from human placenta Sigma-Aldrich Cat #C7521-10MG Vitronectin XF StemCell Technologies Cat #07180 Software GraphPad Prism 10.4 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html DataViewer V2.4.0 https:// www.optics11life.com FlowJo https:// www.flowjo.com Cellpose https:// www.cellpose.org MATLAB https:// www.mathworks.com/ products/matlab.html Affinity Designer https:// affinity.serif.com Other Confocal LSM 710 Carl ZEISS AG Axio Observer 7 Inverted Microscope Carl ZEISS AG ApoTome.2 Fluorescence Microscope Carl ZEISS AG Chiaro Nanoindenter Optics11 Life LSRFortessa Cell Analyser BD Biosciences Dimension Icon Atomic Force Microscope Bruker ..



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